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Double-labeled immunofluorescence of SIRT1/FOXO1 (A), SIRT1/Ac-FOXO1 (B), FOXO1/POMC (C), and Ac-FOXO1/NPY (D) in hypothalamic ARC (* p < 0.05, ** p < 0.01, vs. NG; # p< 0.05, ## p< 0.01, vs. MG; Δ p< 0.05, ΔΔ p< 0.01, vs. EA). SIRT1, silent mating type information regulation 2 homolog 1; FOXO1, forkhead box protein O1; Ac-FOXO1, acetylated FOXO1; NPY, neuropeptide Y; ARC, arcuate nucleus.
