Table 1.
Taxonomic identification, antifungal and antibacterial activity of bacteria from sponge, Suberea mollis.
| Lab no | Accession Number | Similarity with closest type straina | Identityb (%) | Antifungal activityc |
Antibacterial acitivityd |
||||
|---|---|---|---|---|---|---|---|---|---|
| P. capsici | P. ultimum | P. aeruginosa | S. aureus | E. coli | E. faecalis | ||||
| EA345 | KY655446 | Ruegeria arenilitoris G-M8(T) | 99.3 | – | – | – | – | – | – |
| EA346 | KY655447 | Bacillus persicus B48(T) | 98.5 | – | – | – | – | – | – |
| EA347 | KY655448 | Pseudovibrio denitrificans DN34(T) | 99.5 | 3 | 3 | – | – | – | – |
| EA348 | KY655449 | Vibrio sagamiensis LC2-047(T) | 98.1 | 11 | 11 | – | – | – | – |
| EA349 | KY655450 | Pseudovibrio denitrificans DN34(T) | 98.7 | – | – | – | – | – | – |
| EA350 | KY655451 | Bacillus nealsonii DSM15077(T) | 99.4 | – | – | – | – | – | – |
| EA351 | KY655452 | Brachybacterium muris C3H-21(T) | 99.1 | – | – | – | – | – | – |
| EA352 | KY655453 | Vibrio parahaemolyticus NBRC12711(T) | 99 | 6 | 3 | – | – | – | 3 |
| EA353 | KY655454 | Staphylococcus saprophyticus subsp Bovis GTC 843(T) | 100 | 6 | 10 | – | – | – | – |
| EA354 | KY655455 | Bacillus paralicheniformis KJ-16(T) | 99 | 6 | 6 | – | – | – | – |
| EA355 | KY655456 | Pseudovibrio denitrificans DN34(T) | 98.8 | – | – | – | 3 | 3 | 4 |
| EA356 | KY655457 | Ruegeria conchae TW15(T) | 97.7 | – | – | – | – | – | – |
| EA357 | KY655458 | Pseudovibrio denitrificans DN34(T) | 99.2 | 6 | 6 | 3 | |||
| EA358 | KY655459 | Bacillus licheniformis ATCC14580(T) | 97.4 | 4 | 10 | – | – | – | – |
| EA359 | KY655460 | Bacillus subtilis subsp. Inaquosorum KCTC13429(T) | 99.5 | 5 | 11 | – | – | – | – |
| EA360 | KY655461 | Bacillus siamensis KCTC13613(T) | 99.5 | 5 | 5 | – | – | – | – |
| EA361 | KY655462 | Bacillus paralicheniformis KJ-16(T) | 99.3 | 5 | 5 | – | – | – | – |
| EA362 | KY655463 | Bacillus campisalis SA2-6(T) | 99.4 | – | – | – | – | – | – |
| EA363 | KY655464 | Bacillus subterraneus DSM13966(T) | 99.5 | 3 | 3 | – | – | – | – |
| EA364 | KY655465 | Bacillus nealsonii DSM15077(T) | 99.3 | 3 | 3 | – | – | – | – |
| EA365 | KY655466 | Bacillus safensis FO-36b(T) | 99.7 | – | – | – | – | – | – |
| EA366 | KY655467 | Bacillus timonensis 10403023(T) | 98.6 | – | – | – | – | – | – |
| EA367 | KY655468 | Bacillus nealsonii DSM 15077 (T) | 99 | 4 | 3 | – | – | – | – |
| EA368 | KY655469 | Bacillus badius MTCC1458(T) | 99.2 | 3 | 3 | – | – | – | – |
| EA369 | KY655470 | Bacillus endolithicus JC267(T) | 98 | 3 | 3 | – | – | – | – |
| EA370 | KY655471 | Lysinibacillus xylanilyticus DSM 23,493 (T) | 99.3 | 3 | 3 | – | – | – | – |
| EA371 | KY655472 | Bacillus dabaoshanensis GSS04 (T) | 97.5 | 5 | 10 | – | – | – | – |
| EA372 | KY655473 | Ruegeria conchae TW15(T) | 99.4 | 3 | 3 | – | – | – | – |
| EA373 | KY655474 | Micrococcus aloeverae AE-6 (T) | 99.9 | 3 | 3 | – | – | – | – |
a
Identification of bacterial strain based on partial 16S rRNA gene sequence analyses.
b
% similarity of each bacterial strain with closely related type strain.
c
Antagonistic activity of sponge-associated bacteria. The activity was measured after 4–5 days incubation at 28 °C by measuring the clear zone of fungal growth inhibition: −, Negative; +, 3 mm; ++, between 4 and 6 mm; +++, between 7 and 9 mm; ++++, between 10 and 12.
d
Antibacterial activity against human pathogenic bacteria: −, Negative; +, 3 mm. ++, between 4 and 6 mm.