Table 1.
Enzyme in different forms | Total Activity |
Protein | Specific Activity1 |
Purified | Yield | ||
---|---|---|---|---|---|---|---|
(µM)2 | (µM)3 | (mg/mL) | F → G | G → F | Fold | (%) | |
CEE | 1.053 ± 0.002 | 1.064 ± 0.003 | 1.46 ± 0.004 | 0.718 ± 0.008 | 0.726 ± 0.010 | – | 100 |
ASP | 1.332 ± 0.004 | 1.397 ± 0.019 | 1.23 ± 0.002 | 1.080 ± 0.011 | 1.133 ± 0.041 | 1.5 | 84.1 |
EED | 1.503 ± 0.009 | 1.556 ± 0.004 | 0.99 ± 0.001 | 1.509 ± 0.028 | 1.562 ± 0.009 | 2.1 | 67.9 |
PES | 1.550 ± 0.003 | 1.685 ± 0.022 | 0.79 ± 0.014 | 1.943 ± 0.083 | 2.112 ± 0.171 | 2.7 | 54.4 |
CEE-Crude enzyme extract; ASP-Ammonium Sulfate precipitation; EED -Enzyme extract after dialysis; PES-Purified enzyme by Sephacryl S-200.
U/ mg protein.
fru/ml/min.
glu/ml/min.