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. 2019 Dec 23;27(4):1117–1123. doi: 10.1016/j.sjbs.2019.12.024

Table 1.

GI activity in different forms, extracted from S. lividans RSU26.

Enzyme in different forms Total Activity
Protein Specific Activity1
Purified Yield
(µM)2 (µM)3 (mg/mL) F → G G → F Fold (%)
CEE 1.053 ± 0.002 1.064 ± 0.003 1.46 ± 0.004 0.718 ± 0.008 0.726 ± 0.010 100
ASP 1.332 ± 0.004 1.397 ± 0.019 1.23 ± 0.002 1.080 ± 0.011 1.133 ± 0.041 1.5 84.1
EED 1.503 ± 0.009 1.556 ± 0.004 0.99 ± 0.001 1.509 ± 0.028 1.562 ± 0.009 2.1 67.9
PES 1.550 ± 0.003 1.685 ± 0.022 0.79 ± 0.014 1.943 ± 0.083 2.112 ± 0.171 2.7 54.4

CEE-Crude enzyme extract; ASP-Ammonium Sulfate precipitation; EED -Enzyme extract after dialysis; PES-Purified enzyme by Sephacryl S-200.

1

U/ mg protein.

2

fru/ml/min.

3

glu/ml/min.