FIGURE 7.

In vitro and in vivo inhibitory effect of PnBBI on larvae of H. armigera. (A) Comparative inhibitory profiles of PnBBI and SBBI toward bovine trypsin, chymotrypsin and HaTP; (B) Photographs depicting the reduction in size of larvae fed on increasing doses (0.001, 0.0025, and 0.005%) of PnBBI containing diet as compared to larvae fed on control diet at the end of 3, 6 and 9 days of feeding. A set of thirty number of second instar larvae with approximate equal body size and weight were used in the present study for feeding with each concentration of PnBBI; (C) depletion in mean body weights of larvae after feeding on diet containing PnBBI as described above; (D) variation in the activity of midgut HaTPs in larvae after feeding on diet containing PnBBI as described above. The data represented is mean ± SE of three biological replicates. Different lower case letters “a, b, c, and d” indicate statistically different at a significant level (P < 0.05) compared to control.