TABLE 1.

Purification of PnBBI from mature seeds (10 g) of peanut interspecific advanced variety (4368-1).

Purification step	Total protein (mg)	Total activity (TIU)a	Yield recovery (%)	Specific activity (TIU/mg)b	Purification fold change
Crude extract	713	6000	100	8.4	1
(NH4)2SO4 Fractionation (20-60%)	490	5164	86	10.5	1.3
Trypsin-Sepharose 4B	8.4	2500	41.6	298	35.5
Sephadex G-50	1.6	2250	37.5	1406	167

The data shown here is a representative of the purification protocol for PnBBI from three independent biological replicates*. ^a One TI unit is equal to 40-60% inhibition of trypsin enzyme activity by PnBBI. ^bSpecific inhibitory activity is defined as the number of TIU per milligram of PnBBI.*data provided as Supplementary Data Sheet 3.