Fig. 1. OAβ Test via Multimer Detection System. (1) Blood sample is collected and centrifuged. (2) Isolated plasma is spiked with synthetic Aβ42 (10 ng/mL) and incubated at 37°C for 144 hours. (3) Spiked Aβ42 induces Aβ oligomerization, thereby changing the levels of plasma Aβ oligomers. (4) The sample is added to wells that have been precoated by mouse monoclonal antibody 6E10 (capture antibody), which captures Aβ at residues 3-8. (5) FF51-horseradish peroxidase antibody (detection antibody) binds to Aβ residues 1–4. (6) Addition of a chemiluminescent substrate solution results in the formation of a colored product, which can be detected and quantified using a spectrophotometer.

Aβ: amyloid-β, MDS: multimer detection system.