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. 2020 Mar 24;11:226. doi: 10.3389/fphys.2020.00226

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Copyright © 2020 Haidar, O’Neill, Staunton, Bavan, O’Brien, Zouggari, Sharif, Mobasheri, Kumagai and Barrett-Jolley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The correlation between control and NGS expression data. (A) We found a strong correlation between control and treated (72 h of IL1β + TNFα) channel expression data. Each point represents the intersection of four control and four test values. In (B), the correlation between qPCR (ΔΔCts) and NGS ratio (FPKM values) are shown. Data suggest that there only a weak linear correlation between qPCR and NGS. P-value = 0.3. R = 0.29. n = 14 (eight animals for NGS and six animals for PCR). Genes that were upregulated in both datasets are indicated in red. (C) Summary of NGS expression of all known (rat) splice variants of Kcnma1 and associated Kcnmb subunits.