Table 1. Summary table of 2D protein separations of National Institute of Allergy and Infectious Diseases Priority Pathogen agents coupled with mass spectrometry sequencing.
| Species | Purpose | Method | Ref. |
|---|---|---|---|
| Bacillusanthracis | Membrane antigen identificationsEndospore proteome Membrane antigens Vaccine lot comparisons | 2D-PAGE2D-LC2D-PAGE2D-PAGE | [11,42,43,61] |
| Brucellamelitensis | Vaccine vs. wild type strain | 2D-PAGE | [62] |
| Burkholderiacepacia | Quorum sensing mutant vs. parent | 2D-PAGE | [63] |
| Campylobacterjejuni | Planktonic vs. biofilm growth | 2D-PAGE | [64] |
| Coxiellaburnetii | Proteome of lysates | 2D-PAGE | [65] |
| Escherichia coli O157:H7 | Virulent vs. nonvirulent strains | 2D-PAGE | [66] |
| Francisellatularensis | Subspecies strain comparisonsVaccine strain vs. wild type | 2D-PAGE2D-PAGE | [67,68] |
| Listeriamonocytogenes | Exponential vs. stationary phasesCell wall subproteome | 2D-PAGE differential salt extracts | [69,70] |
| Mycobacterium tuberculosis | Aerobic vs. anaerobic growthStrain and method comparisons | 2D-PAGE2D-PAGE/ICAT | [71,72] |
| Rickettsiaprowazekii | Tryptic proteome | 2D-LC | [73] |
| Salmonellatyphimurium | Antibiotic resistancePathogenecity mutantRecombinant protein comparisons | 2D-HPLC2D-PAGE2D-PAGE | [10,74,75] |
| Severe acute respiratory syndrome (coronavirus) | Virions and infected cell lysates | 2D-LC | [76] |
| Toxoplasmagondii | Tachyzoite stage expression | 2D-PAGE | [77] |
| Vibriocholerae | Aerobic vs. anaerobic growthStrain comparisons | 2D-PAGE2D-PAGE | [78,79] |
| Yersiniapestis | Human macrophage response comparison with other Yersiniaspp. | 2D-PAGE | [80] |
HPLC: High-performance liquid chromatography; ICAT: Isotope-coded affinity tag; LC: Liquid chromatography; PAGE: Polyacrylamide gel electrophoresis.