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. Author manuscript; available in PMC: 2020 Mar 31.
Published in final edited form as: Clin Sci (Lond). 2018 Aug 16;132(15):1711–1723. doi: 10.1042/CS20180337

Figure 4. Small interfering RNA (siRNA)-mediated knockdown of IFN-λR1 decreases immunoproteasome subunit expression in rhinovirus-infected human tracheobronchial epithelial (HTBE) cells.

Figure 4.

HTBE cells in submerged culture (n=5 subjects, as represented by different color symbols) were transfected with IFN-λR1 siRNA or Scr siRNA control, and then treated with or without RV16 at MOI 10. Cells were harvested after 24 h of infection to verify knockdown of IFN-λR1 mRNA (A) and protein (B). (B) – LMP2 and LMP7 protein densitometric data and representative Western blot images. (C) – RV load was measured by RT-PCR and was normalized to GAPDH gene. Data were presented as means ± S.E.M. and analyzed using ANOVA.