Table 1.

Top Scaffold Samples That Exhibited the Greatest Displacement of the Fluorescent Probe during Scaffold Screening^a

Scaffold Sample #	Structure	Percent (%) signal change
2408		55.7
2407		21.2
2353		19.5
2157		19.4
2354		16.3

^aScreening results for the mixture-based scaffold ranking library at concentrations of 25 μg mL⁻¹. Scaffold samples were ranked by their average percent change in anisotropy signal relative to controls (free probe in solution = 100%; probe maximally bound to ERK2 = 0%), indicating displacement of the FITC-X-Lig-D probe from the DRS of ERK2. Data reported as the average of two replicate samples at a mixture dose of 25 μg mL⁻¹. Variable functional groups (X_1–5) on each scaffold structure indicate that mixtures of different moieties are present.