Figure 4. Influence of SCFA on the IFNγ production by 7-17 DETC.

7-17 cells were treated with 5 mM butyrate, propionate or acetate and simultaneously stimulated with anti-CD3ε/CD28 antibodies for 6 hours. Subsequently, the supernatants were analyzed for their IFNγ concentrations by ELISA (n = 5; individual biological replicates are connected by lines; two-way ANOVA (biological replicates were paired without Geisser-Greenhouse correction) followed by a Sidak's multiple comparisons test, which compared SCFA-treated samples with controls separately within unstimulated and stimulated samples; ns = not significant