Table 1.
Sequences of siRNAs used for targeting domain III of the internal ribosome entry site of HCV genome
| siRNAa | siRNA sense-stranded (5′→3′) | Degree of conservation (%)b |
|---|---|---|
| si205 | CCCGCUCAAUGCCUGGAGATT | 73.6 |
| si214 | UGCCUGGAGAUUUGGGCGUTT | 73.6 |
| si240-1a | CAAGACUGCUAGCCGAGUATT | 28.9 |
| si240-1b | CGAGACUGCUAGCCGAGUATT | 62.3 |
| si244 | ACUGCUAGCCGAGUAGUGUTT | 71.1 |
| si284 | GUACUGCCUGAUAGGGUGCTT | 98.7 |
| si313 | CCCGGGAGGUCUCGUAGACTT | 98.1 |
| siIRR | CGUUUUACUCCUUAACUUATT | NA |
Abbreviations: HCV, hepatitis C virus; NA, not applicable; siIRR, irrelevant siRNA; siRNA, small interfering RNA.
a
siRNA numbering corresponds to the first nucleotide of the H77 strain of HCV genotype 1a27 targeted by the 21-nucleotide siRNA antisense-strand.
b
Degree of conservation (%) of each siRNA-targeted sequence among 159 HCV sequences of all genotypes, as found in the Los Alamos National Laboratory database (http://hcv.lanl.gov/content/hcv-db/index).