Fig. 2.

Immunoblot of coronavirus nsp1 proteins interacting with ribosomal protein, and lack of IRF-3 phosphorylation inhibition. (A) 293 cells transfected with nsp1-expressing plasmids or control plasmid and immunoprecipitated with anti-HA antibody. Immunoblotting was with anti-S6, anti-HA or anti-β-actin antibodies. Bands for ribosomal 40S subunit, nsp1 and β-actin proteins are shown. (B) 293 cells transfected as in (A) and cell innate immunity stimulated with NDV. Immunoblotting was with anti-phospho-IRF-3 (Ser396), anti-IRF-3 or anti-β-actin antibodies.