Table 1.
Autoantibodies to mouse liver and kidney extracts induced by CCl4 in MHV-infected BALB/c mice.
| Treatment | Serum | Relative mobility (RM) of the autoantigens reacting with the mouse serum(1) |
|
|---|---|---|---|
| Liver(2) | Kidney(2) | ||
| MHV + CCl4 | 1 | 0.4–0.7–1.2 | – |
| 2 | 0.4–0.5–1.8 | ND | |
| 3 | 0.2–0.7–1.1–1.2–1.5 | 0.1–0.3–0.8–1.4–1.6 | |
| 4 | 0.7–1.4 | 1.3 | |
| 5 | 0.1–0.7–1.2 | 0.7–0.9–1.2–1.4 | |
| 6 | 0.1–0.5–0.7 | 0.7–0.9–1.2 | |
| 7 | 0.2–0.3–0.6–0.9 | ND | |
| 8 | – | ND | |
| 9 | – | ND | |
| 10 | 0.7–1.1–1.3–1.6–1.7 | 0.7–0.9–1.2 | |
| 11 | 0.7 | – | |
| MIV | 12 | 0.7 | – |
| 13 | – | 1.2 | |
| 14 | – | 0.5 | |
| 15 | 0.7 | 0.7–1.2 | |
| 16 | – | – | |
| 17 | – | – | |
Tissue extracts, separated by SDS-PAGE in 10% gels, were transferred onto nitrocellulose sheets and incubated with 1:50 serum dilution. Bound Ab were revealed by peroxidase-labeled IgG anti-mouse IgG and ECL reagents (see Materials and methods).
(1) Relative Mobilities (RM) of the autoantigens were calculated as explained in Fig. 2A.
(2) RM values 0.6–0.8 correspond to the FAH protein.
ND: not done.