Table 1.
Broad-range primers for bacteria used for PCR/ESI-MS.
| Gene target | Bacterial detection | Primer pairs⁎⁎ |
|---|---|---|
| 16S rDNA | Universal | 346,347,348,361 |
| 23S rDNA | Universal | 349,360 |
| rpoC | Bacteroidetes, fusobacteria, spirochaetes, bacilli, proteobacteria (α/β/γ) | 354 |
| rpoC | Proteobacteria (α/β) | 363 |
| valS | Some γ-proteobacteria: Erwinia | 358 |
| rpoB | Pantoea, Pectobacterium | 359 |
| rpoB | Proteobacteria (α/β) | 362 |
| tufB | Some β-proteobacteria: Eikenella, Neisseria, Achromobacter, Bordetella, Burkholderia, Ralstonia | 367 |
| rplB | Clostridia, fusobacteria, bacilli, and ε-proteobacteria (Campylobacter, Helicobacter, Wolinella) | 356, 449 |
| infB | Bacilli | 352 |
| sspE | Bacillus cereus | 355 |
⁎⁎
Adapted from Baldwin et al. (2009). For specific primer sequences see Hofstadler et al. (2005).