Table 1.
Gene target | Bacterial detection | Primer pairs⁎⁎ |
---|---|---|
16S rDNA | Universal | 346,347,348,361 |
23S rDNA | Universal | 349,360 |
rpoC | Bacteroidetes, fusobacteria, spirochaetes, bacilli, proteobacteria (α/β/γ) | 354 |
rpoC | Proteobacteria (α/β) | 363 |
valS | Some γ-proteobacteria: Erwinia | 358 |
rpoB | Pantoea, Pectobacterium | 359 |
rpoB | Proteobacteria (α/β) | 362 |
tufB | Some β-proteobacteria: Eikenella, Neisseria, Achromobacter, Bordetella, Burkholderia, Ralstonia | 367 |
rplB | Clostridia, fusobacteria, bacilli, and ε-proteobacteria (Campylobacter, Helicobacter, Wolinella) | 356, 449 |
infB | Bacilli | 352 |
sspE | Bacillus cereus | 355 |
Adapted from Baldwin et al. (2009). For specific primer sequences see Hofstadler et al. (2005).