Table 2.
Comparison of Analysis of 1 Million versus 10 Million Reads
| Virus | Virus family | Cq value | 10 million reads |
1 million reads |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Total reads | Virus family reads | % of total | % of viral | Total reads | Virus family reads | % of total | % of viral | |||
| RV | Picornaviridae | 37.7 | 8,203,894 | 8941 | 0.06 | 84.37 | 822,218 | 889 | 0.07 | 86.11 |
| PIV4 | Paramyxoviridae | 24.9 | 10,886,798 | 2136 | 0.04 | 41.90 | 1,088,067 | 199 | 0.08 | 40.73 |
| CMV | Herpesviridae | 34.5 | 15,889,428 | 22 | 00.01 | 10.88 | 1,588,922 | 2 | 0.04 | 11.87 |
| ADV | Adenoviridae | 30.2 | 11,146,488 | 0 | 0 | 0 | 1,115,135 | 0 | 0.03 | 0 |
| RSV | Pneumoviridae | 27.3 | 10,191,995 | 1477 | 0.02 | 53.29 | 1,019,415 | 163 | 0.04 | 59.25 |
| INFB | Orthomyxoviridae | 30 | 8,535,672 | 652 | 0.01 | 48.67 | 853,149 | 61 | 0.02 | 46.58 |
| NL63 | Coronaviridae | 36.2 | 10,386,928 | 0 | 0 | 0 | 1,038,469 | 0 | 0.02 | 0 |
| INFA | Orthomyxoviridae | 27.5 | 10,981,601 | 8403 | 0.11 | 70.28 | 1,097,872 | 855 | 0.17 | 69.84 |
| MPV | Pneumoviridae | 34.1 | 12,972,626 | 2 | 0 | 0.10 | 1,297,151 | 0 | 0.02 | 0 |
| HBOV | Parvoviridae | 32.2 | 11,819,805 | 0 | 0 | 0 | 1,181,738 | 0 | 0 | 0 |
| RV | Picornaviridae | 23.1 | 11,819,805 | 58,695 | 0.42 | 84.27 | 1,183,738 | 5754 | 0.49 | 84.25 |
% of total, percentage of total reads; % of viral, percentage of all viral reads; ADV, adenovirus; CMV, cytomegalovirus; Cq, quantification cycle; HBOV, human bocavirus; INFA, influenza A virus; INFB, influenza B virus; MPV, metapneumovirus; NL63, coronavirus NL63; PIV4, parainfluenza virus 4; RSV, respiratory syncytial virus; RV, rhinovirus.