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. 2019 Dec 16;22(2):196–207. doi: 10.1016/j.jmoldx.2019.10.007

Table 2.

Comparison of Analysis of 1 Million versus 10 Million Reads

Virus Virus family Cq value 10 million reads
1 million reads
Total reads Virus family reads % of total % of viral Total reads Virus family reads % of total % of viral
RV Picornaviridae 37.7 8,203,894 8941 0.06 84.37 822,218 889 0.07 86.11
PIV4 Paramyxoviridae 24.9 10,886,798 2136 0.04 41.90 1,088,067 199 0.08 40.73
CMV Herpesviridae 34.5 15,889,428 22 00.01 10.88 1,588,922 2 0.04 11.87
ADV Adenoviridae 30.2 11,146,488 0 0 0 1,115,135 0 0.03 0
RSV Pneumoviridae 27.3 10,191,995 1477 0.02 53.29 1,019,415 163 0.04 59.25
INFB Orthomyxoviridae 30 8,535,672 652 0.01 48.67 853,149 61 0.02 46.58
NL63 Coronaviridae 36.2 10,386,928 0 0 0 1,038,469 0 0.02 0
INFA Orthomyxoviridae 27.5 10,981,601 8403 0.11 70.28 1,097,872 855 0.17 69.84
MPV Pneumoviridae 34.1 12,972,626 2 0 0.10 1,297,151 0 0.02 0
HBOV Parvoviridae 32.2 11,819,805 0 0 0 1,181,738 0 0 0
RV Picornaviridae 23.1 11,819,805 58,695 0.42 84.27 1,183,738 5754 0.49 84.25

% of total, percentage of total reads; % of viral, percentage of all viral reads; ADV, adenovirus; CMV, cytomegalovirus; Cq, quantification cycle; HBOV, human bocavirus; INFA, influenza A virus; INFB, influenza B virus; MPV, metapneumovirus; NL63, coronavirus NL63; PIV4, parainfluenza virus 4; RSV, respiratory syncytial virus; RV, rhinovirus.