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Schematic representation of NASBA. RNA (red wavy line) is converted to double-stranded DNA with a T7 promoter using reverse transcriptase, RNaseH and a primer with a T7 promoter. The DNA is used as a template by T7 RNA polymerase for the production of multiple copies of antisense RNA (black wavy lines). Each transcript can act as a template for the production of additional double-stranded DNA templates.
