Figure 4.

Treatment with PLGA/CaP NPs reduces lung inflammation. During induction of inflammation, mice were treated with siRNA-loaded CaP/PLGA NPs directed against CCL2, IP-10 and IFN-γ by nasal instillation on days 1, 3 and 5 (A). On day 6, mice were analyzed for weight loss (B), and the infiltration of cells into the lung (C). The histopathology in the lung was analyzed (D) and expression levels of target genes in lung tissue or BALF were measured by qPCR and Luminex assay (E). Data from two independent experiments are shown. Statistical analysis was performed with one-way ANOVA followed by Tukey's multiple comparison test or Student's t-test (*p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant).