Fig. 1.

A. Survival rate of mice orally challenged with 0.4 mL of viable S. typhimurium organisms (10⁹ CFU mL^− 1 per mouse) at 0 and 24 h. Unchallenged mice received no treatment, whereas the challenged mice were treated once a day (after the 2nd bacterial challenge) for 7 consecutive days with 0.4 mL of 20 mg mL^− 1 specific IgY, 0.4 mL of 20 mg mL^− 1 nonspecific IgY or 0.4 mL of PBS (n = 15 per group). Data were analyzed by the Chi-square test. *P < 0.05 compared with no treatment.

B. Weight gain of unchallenged mice receiving no treatment, untreated mice challenged with S. typhimurium and challenged mice treated with 0.4 mL of 20 mg mL^− 1 nonspecific IgY, 0.4 mL of 20 mg mL^− 1 nonspecific IgY or 0.4 mL of PBS 7 days post-challenge (n = 6 per group). Data are expressed as means ± SD and analyzed by ANOVA followed by SNK. Means without the same letter are significantly different (P < 0.05).