Skip to main content
. 2012 May 2;13(3):331–340. doi: 10.1016/j.intimp.2012.03.011

Supplement Fig. 2.

Supplement Fig. 2

The expression of IFN-γ, IL-4, TGF-β1 and TGFβRI in lung tissues assessed using an immunohistochemical DAB stain. The arrow indicates a positive cell.

IFN-γ: LPS group on day 7 (A, × 400): there were large numbers of positive cells varying in shape in the alveolar cavity and alveolar spaces; day 28: there were fewer positive cells, which were mainly located in the interstitial alveolar spaces. Dex group on day 7: fewer positive cells located in the pulmonary cavity; day 28: the numbers of positive cells increased and were located in the alveolar spaces. H-HC group on day 7: there were many positive cells in the alveolar cavity and the alveolar spaces; day 28, fewer positive cells were found in the alveolar spaces.

IL4 in LPS group on day 7 (G, × 400): there were a high number of positive cells; day 28: fewer positive cells, which were primarily located in the interstitial alveolar spaces. IL-4 in Dex group on day 7: there were fewer positive cells; day 28: the numbers of positive cells decreased, and the positive cells were primarily located in the alveolar spaces. IL-4 in H-HC group on day 7: there were some positive cells in the alveolar cavities and alveolar spaces; day 28: fewer positive cells, which were mainly located in the alveolar spaces.

TGF-β1: LPS group on day 7 (G, × 400): high expression of positive cells; day 28: the number of positive cells was decreased, and the positive cells were mainly located in the interstitial alveolar spaces. Dex group on day 7: there were fewer positive cells; day 28: the number of positive cells increased slightly, and most of them were inflammatory cells in the alveolar cavities. H-HC group on day 7: there were many positive cells in the alveolar cavities and alveolar spaces; day 28: the expression of positive cells was decreased, and the positive cells were mainly located in alveolar spaces.

TGFβRI: LPS group on day 7 (S, × 400): there was a large number of positive cells, and most of the positive cells were mononuclear phagocytes located in the alveolar spaces; day 28: the number of positive cells decreased. Dex group on days 7 to 28: there were fewer positive cells, and most of them were inflammatory cells located in the alveolar cavities. H-HC group on days 7 to 28: there were some positive cells; day 28: the positive cells were mainly located in the alveolar spaces.