Fig. 1.

Determination of Akt phosphorylation in A549 cells. (A)–(C) Serum-starved A549 cells were infected with live MPC/04 (A) at an MOI of 5 (B) with UV-treated MPC/04 or (C) with mock virus. The phosphorylated Akt and total Akt levels were examined at the indicated times points by western blots. (D) and (E) Serum-starved A549 cells were pre-incubated with LY294002 (LY; 10–50 μM) (D), Wortmannin (Wort; 100 nM–1 μM) (E) or DMSO (0.4%, v/v) for 1 h and subsequently infected with MPC/04 at an MOI of 5. A mock-infected lane was included in each panel as a negative control. After 30 min, the phosphorylated Akt and total Akt levels were examined by western blots. (F) A549 cells (10⁵) were transfected with 50 nM siRNA targeting p85 or negative siRNA, and then infected with MPC/04 at an MOI of 5 at 24 h after transfection. The expression of p-Akt, total Akt, p85 and GAPDH was determined 30 min post-infection. The data represent the results of three independent experiments.