Fig. 2.

Blocking the PI3K/Akt pathway promotes reovirus replication. (A)–(D) Serum-starved A549 cells were pre-incubated with increasing concentrations of LY294002 (A and C), Wortmannin (B and D) or DMSO for 1 h and then infected with MPC/04 at an MOI of 5. Twenty-four hours p.i., the relative expression of the viral S4 gene was analyzed by real-time PCR (A and B), and the cell supernatants were harvested for an analysis of the viral titers (C and D). (E) and (F) Serum-starved A549 cells were inoculated with LY294002 (50 μM) or DMSO at the indicated time points during the infection of MPC/04 at an MOI of 5. Twenty-four hours after infection, the relative expression of the S4 gene was analyzed by real-time PCR (E), and the cell supernatants were harvested for an analysis of the virus titers (F). The data represent the results of three independent experiments. ^*p < 0.05, ^**p < 0.001. Error bars indicate SD.