Table 4.
Antigenic specificities of coronavirus-myelin cross-reactive TCC
| Clones | HCoV-229E | HCoV-OC43 | MBP | PLP |
|---|---|---|---|---|
| P7a | NS | 5.9 (4772) | 2.4 (1584) | NS |
| P8a | 2.7 (5000) | NS | 3.5 (1231) | NS |
| P12a | 3.1 (2300) | NS | NS | 3.2 (1198) |
| P12b | 4.5 (1542) | NS | NS | 5.2 (985) |
| P12c | 2.9 (1120) | NS | 3.4 (898) | NS |
| P13a | 11.3 (4293) | NS | 4.2 (4233) | NS |
| P13b | NS | 9.0 (10800) | 8.4 (7365) | 3.4 (2400) |
| P22a | 18.3 (6284) | NS | 3.8 (2628) | NS |
| P22b | 9.0 (2322) | NS | 4.9 (1014) | 5.1 (1164) |
| P24a | 18.0 (9020) | NS | 3.4 (1205) | NS |
The first number indicates the stimulation index (S.I.) and CPM counts are indicated in parentheses. The S.I. for a given antigen is the ratio of the radioactive CPM obtained after tritiated-thymidine incorporation when the T cells are stimulated with the selecting antigen over the CPM generated by stimulation with the corresponding control antigen. A T-cell clone was considered positive for an Ag if the CPM count was higher than 800 and if the S.I. was of 2 or more. The values in bold indicate the selecting Ag for a specific TCC. During antigenic specificity assays, viral Ag was tested against myelin Ag and vice versa. Viral and myelin Ag were not tested against one another. The name of the cross-reactive TCC in bold indicates the TCC that were further studied and sequenced. Proliferation that did not reach our criteria is considered non-significant (NS).