Table 5.
Characterization of the TCRVβ chains
| TCC | HLA-DR typing | Antigenic specificity | Vβ chain | V | N-D-N | J | |
|---|---|---|---|---|---|---|---|
| P7a | 11, 17 | OC43 | MBP | 6S7*2 | CASS | LNPASYG | EQYVGPGTM |
| P8a | 15, 17 | 229E | MBP | 6S5 | CASS | LSLYSG | GNETRLGT |
| P12a | 13 | 229E | PLP | 6S7*2 | CASS | SLNGSPL | GPGTRLTVT |
| P13a | 15 | 229E | MBP | 6S5 | CASS | LGPASYE | GPGTRLTVT |
| P13b | 15 | OC43 | MBP/PLP | 8S2 | CASS | LSLLSPH | GPGTRLTVT |
Following RACE RT-PCR amplification, the Vβ chains of five cross-reactive TCC were sequenced in order to identify the hypervariable CDR3 region. Here the HLA-DR is indicated and the variable region (V), the hypervariable portion N-D-N and the joining (J) region are characterized.