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. 2006 Jun 16;120(2):171–178. doi: 10.1016/j.clim.2006.05.002

Figure 1.

Figure 1

SARS-CoV S-peptide-specific IFN-γ production by PBMCs. Fresh PBMCs were isolated from fully recovered SARS patients and normal individuals and cultured in vitro with or without a pool of 169 SARS-CoV S peptides in the presence of anti-CD28 (1 μg/ml) and anti-CD49d mAbs (1 μg/ml). After incubation for 72 h, the level of IFN-γ in culture supernatants was assayed by ELISA (A). The frequency of IFN-γ-secreting cells was detected by ELISpot assay at the single cell level after stimulation for 20 h (B). Student's t test was used for statistical analysis. The significance was set at P < 0.01 (***). ns, not significant.