Table 3.
Performance of Real-Time RT-PCR Assay on Serum Specimens
| Type of serum samples | No. of samples | No. of samples correctly serotyped | Serotyping sensitivity, % (95% CI)∗ | Detection sensitivity, % (95% CI)† | Detection specificity, % (95% CI)‡ |
|---|---|---|---|---|---|
| DENV-1 | 29 | 26 | 89.66 (71.50– 97.29) | 100 (93.58–100) | 100 (85.87–100) |
| DENV-2 | 30 | 29 | 96.67 (80.95– 99.83) | ||
| DENV-3 | 30 | 29 | 96.67 (80.95– 99.83) | ||
| DENV-4 | 17 | 16 | 94.12 (69.24– 99.69) | ||
| CHIKV | 47 | 45 | 95.74 (84.27–99.26) | 100 (85.87–100) | |
| Healthy samples | 30 | 0 |
∗
Number of specimens with correct serotype detected/(number of specimens with correct serotype detected + number of specimens with wrong serotype detected) × 100%.
†
Number of positive specimens/(number of positive specimens + number of false-negative specimens) × 100%.
‡
Number of negative specimens/(number of negative specimens + number of false-positive specimens) × 100%.