Fig. 1.

(A) Indirect immunofluorescence detection of MRV in Vero E6 cells infected with strain MPC/04. The cells were fixed at 4% paraformaldehyde, blocked with 1% BSA, washed, and incubated with mouse anti-MRV (T3D) antibody. The cells were then incubated with FITC-labeled goat anti-mouse IgG secondary antibody. (B) Electron micrographs. a: negative staining of cell-culture supernatant. Non-enveloped reoviral particles with double-layered capsid structure were observed (diameter = approximately 70 nm). b: ultra-thin sections of infected Vero E6 cells displayed typical contrast-rich virus particles, organized as paracrystalline structures within the cytosol.