Figure 7.
Contribution of FBL Overexpression to Cancer Phenotype
(A) Cell proliferation of three each independent stable MCF7 clones expressing FBL-GFP (F1, F2, and F3) or GFP (G1, G2, and G3).
(B) Proliferation of the indicated cell clones not treated (NT) or treated with 1 μM Osi-906 for 72 hr.
(C and D) Anchorage-independent growth of MCF7 clones using soft agar assay. Representative images are shown in (C) and the numbers of colonies determined in three experiments are shown in (D).
(E) Impact of FBL overexpression on drug response was investigated by determining the IC50 of doxorubicin using MTS assays. Error bars represent SD. The p values have been determined by a Mann-Whitney W test.
(F and G) Kaplan-Meier analysis of relapse-free survival rates (event = relapse) (F) and of breast cancer-specific survival rates (event = death related to breast cancer disease) (G) according to FBL mRNA level in primary breast tumor samples. The data are dichotomized at the upper quartile value into high and low expression groups.