Table 1.

Oligonucleotide primers used for IBV genome amplification and their positions in the genome.

Primer	Sensea	Sequence (5′ to 3′)	Position in genomeb
IBV-366	+	AACCCAAAAGATTACGCTGATGCTT	640–664
IBV-367	−	CCAGAATTCAAAAGACTTTTCAGTA	1887–1911
IBV-368	+	GATGTCTTGAAGCTGTTTCAATC	1828–1850
IBV-369	−	AAGAATGTGCTTGTAGATGGCGT	3817–3839
IBV-370	+	TATGTTAAGAAACATGGGCCAC	3685–3706
IBV-371	−	TACATTTATGTCTCCTGAAGTTGCTGG	6405–6431
IBV-372	+	GTAAGAGACATAATTGGTATTG	6353–6374
IBV-373	−	GGTGCAGTTTTAATTTTACAAACTGC	9052–9077
IBV-377	+	CTTAATCTTGCTAATAATCATGAG	8971–8994
IBV-378	−	GTGTACAACTGTTGTTTTAATGCAGC	10,686–10,711
IBV-379	+	GTGTGGGAAGTCTTTTCGACAAATATAC	10,588–10,615
IBV-380	−	GGACGTTGTCAATTTAAAGGTTC	12,100–12,122
IBV-381	+	CTCCTGATCAGGATTCTTATGGAGGAGC	12,011–12,038
IBV-382	−	CCTGTGGCTGTTATGGAGCGTTATAT	14,835–14,860
IBV-383	+	CATTTTGGGTGCATGTGTTTTTGTAGATG	14,789–14,817
IBV-384	−	GACACCATGTGACAAACACTACACAATCTG	17,407–17,436
IBV-375	+	CCTTGGCATGTTATAAGACCAAGGATAG	17,346–17,373
IBV-376	−	CGCTCCATCTTTTAATGGCATACCATTCTG	18,903–18,932
IBV-374	+	GTTCTGTATGATGATAGATATGGTGATTACC	18,780–18,810
IBV-105-1	−	CCAAAGTGCCTTTAGACCGGCTG	20,388–20,412
IBV-257	+	TATTGATTAGAGATGTGG	20,356–20,373
IBV-275	−	GTTACAGATGAGTACATAC	22,147–22,165
IBV-167	+	GCTTCTTGAGAA(T/C)CAGTTTTA	21,921–21,941
IBV-168	−	AGACGATCAACTTGTGCATCTG	22,952–22,973
IBV-182	+	GACATTTAC(C/G)(A/C)GCAACTTGA	22,921–22,940
IBV-281	−	GCCACTGACC(C/A)TCACAATAAAG	24,955–24,976
IBV-280	+	CCC(C/A)GAATCTAATGCCGTAGG	24,846–24,866
IBV-171	−	AACCAAGATGCATTTCCAGA	25,960–25,979
N(+)	+	GACGCCCCAGCGCCAGTCATTAAA	25,903–25,926
N(−)c	−	ACGCGGAGTACGATCGAGGGTACA	27,484–27,507

^aNegative-sense (−) or positive-sense (+) primer.

^bNucleotide positions corresponded to the sequence of the IBV Beaudette genome, GenBank accession number M95169.

^cPrimer N(−) was used for RT.