Table 3.
Intra- and Interassay Variability of MERS-CoV ORF1a and upE RT-iiPCR
| Assay run | RT-iiPCR result∗ (positive/total) | S/N (average ± SD) |
|---|---|---|
| MERS-CoV ORF1a RT-iiPCR | ||
| 1 | 3/3 | 4.95 ± 0.03 |
| 2 | 3/3 | 4.92 ± 0.02 |
| 3 | 3/3 | 4.85 ± 0.11 |
| MERS-CoV upE RT-iiPCR | ||
| 1 | 3/3 | 4.94 ± 0.05 |
| 2 | 3/3 | 4.85 ± 0.05 |
| 3 | 3/3 | 4.93 ± 0.05 |
MERS-CoV, Middle East respiratory syndrome–coronavirus; PFU, plaque forming units; RT-iiPCR, reverse transcription-insulated isothermal PCR; S/N, signal-to-noise ratio; TCF, tissue culture fluid.
∗
Nucleic acid extract of 10−5 dilution (3.7 × 10−1 PFU/mL) of MERS-CoV infectious TCF.