. 2017 Aug 12;19(6):817–827. doi: 10.1016/j.jmoldx.2017.06.007

Table 4.

Detection of MERS-CoV by RT-iiPCR and RT-qPCR in Sputum Samples Collected from Patients Infected with MERS-CoV

Patient's ID	Age	Sex	Date of onset	Outcome	No. of samples	Collection date^∗	Initial laboratory testing (RT-PCR)^†	MERS-CoV RT-iiPCR		MERS-CoV RT-qPCR		Estimated viral load^‡ (log₁₀ [copies])		Internal control (C_T)^§
Patient's ID	Age	Sex	Date of onset	Outcome	No. of samples	Collection date^∗	Initial laboratory testing (RT-PCR)^†	ORF1a	upE	ORF1a	upE	ORF1a	upE	RNase P
P016	40	M	05-19-2015	Recovered	8	06-09-2015	+	+	+	+	+	3.906	4.195	35.8
						06-10-2015	+	+	+	+	+	2.681	3.111	32.8
						06-10-2015′	+	+	+	+	+	3.507	3.289	32.2
						06-11-2015	+	+	+	+	+	6.024	6.482	30.9
						06-11-2015′	+	+	+	+	+	5.779	6.224	32.1
						06-13-2015	+	+	+	+	+	5.266	5.837	31.9
						06-14-2015	+	+	+	+	+	2.363	2.846	32.3
						06-14-2015′	+	+	+	+	+	2.478	3.128	32.7
P030	60	M	05-30-2015	Recovered	6	06-08-2015	+	+	+	+	+	6.527	7.049	29.7
						06-09-2015	+	+	+	+	+	4.936	5.132	28.4
						06-09-2015′	+	+	+	+	+	4.917	5.243	29.7
						06-11-2015	+	+	+	+	+	6.017	6.406	27.4
						06-11-2015′	+	+	+	+	+	5.986	6.360	28.4
						06-11-2015″	+	+	+	+	+	6.238	6.521	24.9
P031	69	M	05-30-2015	Fatal	5	06-08-2015	+	+	+	+	+	6.821	6.864	27.8
						06-11-2015	+	+	+	+	+	7.878	8.242	28.2
						06-12-2015	+	+	+	+	+	5.996	6.416	34.1
						06-15-2015	+	+	+	+	+	6.478	6.125	25.3
						06-17-2015	+	+	+	+	+	5.322	5.003	24.6
P054	62	F	05-31-2015	Recovered	4	06-12-2015	+	+	+	+	+	6.306	6.447	31.4
						06-14-2015	+	+	+	+	+	6.701	7.025	28.4
						06-15-2015	+	+	+	+	+	5.672	5.700	27.1
						06-16-2015	+	+	+	+	+	5.983	5.812	31.9
P082	82	F	06-04-2015	Fatal	3	06-11-2015	+	+	+	+	+	2.716	4.159	31.0
						06-13-2015	+	+	+	+	+	6.146	6.719	26.3
						06-14-2015	+	+	+	+	+	3.756	4.600	27.9
P085	65	F	06-06-2015	Recovered	5	06-10-2015	+	+	+	+	+	4.347	4.100	26.6
						06-13-2015	+	+	+	+	+	5.049	5.138	35.0
						06-14-2015	+	ND	+	ND	ND	ND	ND	27.3
						06-15-2015	+	+	+	+	+	4.376	4.590	29.5
						06-16-2015	+	+	+	+	+	3.842	4.241	31.8
P110	57	F	06-02-2015	Recovered	2	06-13-2015	+	+	+	+	+	5.538	5.491	29.7
P110	57	F	06-02-2015	Recovered	2	06-13-2015′	+	+	+	+	+	5.040	5.411	27.5
P122	55	F	06-06-2015	Recovered	6	06-10-2015	+	+	+	+	+	6.168	6.731	29.2
						06-12-2015	+	+	+	+	+	5.075	5.221	34.5
						06-13-2015	+	+	+	+	+	5.218	5.750	31.7
						06-14-2015	+	+	+	+	+	4.866	5.177	29.5
						06-15-2015	+	+	+	+	+	3.100	3.401	29.8
						06-16-2015	+	+	+	+	+	3.702	3.789	33.2
P130	65	F	06-12-2015	Recovered	2	06-14-2015	+	+	+	ND	+	ND	2.672	30.2
P130	65	F	06-12-2015	Recovered	2	06-16-2015	+	+	+	+	+	2.160	2.604	30.2
P148	38	F	06-06-2015	Recovered	4	06-15-2015	+	+	+	+	+	3.564	3.678	35.8
						06-17-2015	+	+	+	+	+	5.781	6.081	33.1
						06-18-2015	+	+	+	+	+	5.516	5.843	31.3
						06-21-2015	+	+	+	ND	+	ND	2.743	38.5
P172	60	F	06-15-2015	Recovered	10	06-22-2015	+	+	+	+	+	5.359	5.812	29.8
						06-25-2015	+	+	+	+	+	3.936	4.074	28.0
						06-25-2015	+	+	+	+	+	4.614	4.722	28.3
						06-27-2015	+	+	+	+	+	3.133	3.007	28.0
						06-28-2015	+	+	+	+	+	4.949	5.153	26.0
						06-28-2015′	+	+	+	+	+	4.559	4.922	26.4
						06-29-2015	+	+	+	+	+	3.961	4.157	28.2
						06-29-2015′	+	+	+	+	+	2.923	3.328	26.9
						06-29-2015″	+	+	+	+	+	2.710	3.308	25.8
						06-30-2015	+	+	+	+	+	5.497	5.791	27.8

F, female; M, male; +, positive; MERS-CoV, Middle East respiratory syndrome–coronavirus; ND, not detected; RT-iiPCR, reverse transcription-insulated isothermal PCR.

^∗

Dates indicated with (′) and (′′) indicate sample replicate.

^†

Patients were confirmed positive by real-time RT-qPCR at the Korean Center for Disease Control laboratory during the 2015 outbreak.

^‡

Viral loads (Log₁₀ copies) were quantified by using in vitro transcribed RNA derived from the amplicon region of each assay.

^§

All specimens were tested for the human RNase P gene to monitor nucleic acid extraction efficiency and the presence of PCR inhibitors as described in Lu et al.³⁴