Table 5.
Diagnostic Performance Comparison between MERS-CoV RT-iiPCR and RT-qPCR Assays
| ORF1a | MERS-CoV RT-qPCR |
||
|---|---|---|---|
| Positive | Negative | Total | |
| MERS-CoV RT-iiPCR | |||
| Positive | 52 | 2 | 54 |
| Negative | 0 | 49 | 49 |
| 52 | 51 | 103 | |
| Agreement (95% CI): 98.06% (94.43%–100%); κ = 0.96 | |||
|
upE |
MERS-CoV RT-qPCR |
||
| Positive |
Negative |
Total |
|
| MERS-CoV RT-iiPCR | |||
| Positive | 54 | 1 | 55 |
| Negative | 0 | 48 | 48 |
| 54 | 49 | 103 | |
| Agreement (95% CI): 99.03% (95.88%–100%); κ = 0.99 | |||
MERS-CoV, Middle East respiratory syndrome–coronavirus; RT-iiPCR, reverse transcription–insulated isothermal PCR; RT-qPCR, real-time quantitative RT-PCR.