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Histological analysis of murine precision cut lung tissue slices (PCLTS) by H & E staining. At day 5 of culture, PCLTS show typical and well-preserved lung tissue structure; alveolar space are delimited by fine alveolar septa (1A, 100× magnification), pneumocytes type I (asterisks) are observed as squamous epithelial cells and pneumocytes type II as cuboidal cells (arrows) at the angles where alveolar septal walls converge (1B, 400× magnification).
