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Acid-fast staining of PCLTS infected with M. tuberculosis at 24 h post infection. Bacteria (arrows) are in contact with pneumocytes type II (NII) (panel A). Bacilli are also localized near alveolar septa and a macrophage is detected in the lumen of the alveolus (panel B). It was common to find mycobacteria on the surface of the epithelium (panel C) and inside alveolar macrophages (panel D). All sample were Ziehl-Neelsen stained. Magnification: 1000×.
