Fig. 5.

Niclosamide inhibits both HIV and mycobacterial growth in infected macrophages. Monocyte-derived macrophages were infected with HIV JR-CSF in RPMI at MOI of 0.05 for 5 h, then washed in PBS and infected with M. bovis BCG-tdTomato in RPMI at MOI of 5 for 24 h. Macrophages were then cultured in the presence or absence of 1.25 μM niclosamide for 6 days. Cells were harvested, stained with a LIVE/DEAD marker, fixed and permeabilized, then stained with an anti-HIVp24-FITC antibody and analyzed by flow cytometry. Niclosamide reduced the replication of both pathogens by over 50% without significant cell loss due to drug toxicity. Data shown is representative of three separate experiments with similar results.