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. 2018 Oct 9;308(8):1113–1120. doi: 10.1016/j.ijmm.2018.10.001

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Fig. 4 — nanoESI mass spectra of sialic acids obtained from digests of BSM treated with BTSA-His alone (A), BTSA-His combined with NanS-His (B), and BTSA-His combined with NanS-p1-His (C). The nanoESI MS analysis revealed ions which could be assigned to Neu5Ac(OAc) and Neu5Gc(OAc) only in the digests when using BTSA-His alone (A), whereas the combined digests (B and C) solely revealed the Neu5Ac and Neu5Gc cores. Ac, Neu5Ac; Ac(OAc), Neu5Ac(OAc); Gc, Neu5Gc; Gc(OAc), Neu5Gc(OAc). The numbered ions in the mass spectra depicted in panel A, B, and C, are listed in Table 3A–C, respectively.