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. 2018 Oct 9;308(8):1113–1120. doi: 10.1016/j.ijmm.2018.10.001

Table 2.

Detected ions derived from chemically released sialic acids of BSM without enzyme (A), NanS-His treated BSM (B), and NanS-p1-His treated BSM (C), and corresponding m/z values.

No. Sialic acid species m/z No. Sialic acid species m/z
A
1 Neu5Ac+ -H2O 274.10 10 Neu5Gc(OAc)+ -H + Na 372.09
2 Neu5Ac+ -H + Na 314.09 11 Neu5Ac(OAc) [M + Na]+ 374.11
3 Neu5Ac(OAc)+ -H2O 316.11 12 Neu5Ac(OAc)2+ 376.13
4 Neu5Gc+ -H + Na 330.08 13 Neu5Gc(OAc) [M + Na]+ 390.10
5 Neu5Ac [M + Na]+ 332.10 14 Neu5Ac(OAc)2+ -H + Na 398.11
6 Neu5Ac(OAc)+ 334.12 15 Neu5Ac(OAc)2 [M + Na]+ 416.12
7 Neu5Gc [M + Na]+ 348.09 16 Neu5Ac(OAc)3 [M + Na]+ 458.13
8 Neu5Ac(OAc)+ -H + Na 356.10 17 Neu5Gc(OAc)3 [M + Na]+ 474.13
9 Neu5Ac(OAc)2+ -H2O 358.11



B
1 Neu5Ac+ -H2O 274.10 9 Neu5Gc(OAc)+ -H + Na 372.09
2 Neu5Ac+ -H + Na 314.09 10 Neu5Ac(OAc) [M + Na]+ 374.11
3 Neu5Gc+ -H + Na 330.08 11 Neu5Ac(OAc)+ -2H+2Na 378.08
4 Neu5Ac [M + Na]+ 332.10 12 Neu5Gc(OAc) [M + Na]+ 390.10
5 Neu5Ac+ -2H+2Na 336.07 13 Neu5Gc(OAc)+ -2H+2Na 394.07
6 Neu5Gc [M + Na]+ 348.09 14 Neu5Ac(OAc)2+ -H + Na 398.11
7 Neu5Gc+ -2H+2Na 352.06 15 Neu5Gc(OAc)2+ -H + Na 414.09
8 Neu5Ac(OAc)+ -H + Na 356.10 16 Neu5Ac(OAc)2 [M + Na]+ 416.12



C
1 Neu5Ac+ -H2O 274.10 7 Neu5Gc [M + Na]+ 348.09
2 Neu5Ac+ -H + Na 314.09 8 Neu5Ac(OAc)+ -H + Na 356.10
3 Neu5Ac(OAc)+ -H2O 316.11 9 Neu5Gc(OAc)+ -H + Na 372.09
4 Neu5Gc+ -H + Na 330.08 10 Neu5Ac(OAc) [M + Na]+ 374.11
5 Neu5Ac [M + Na]+ 332.10 11 Neu5Gc(OAc) [M + Na]+ 390.10
6 Neu5Ac(OAc)+ 334.12 12 Neu5Ac(OAc)2+ -H + Na 398.11