Table 1.

HLA-A2/A0201-restricted T-cell epitopes of HIV p24 protein identified by at least 4 epitope prediction methods and molecular docking technique.

Peptide	Sequence	No. of methods	Methods^a	Peptide binding free energy score			Epitope
				MHC	TCR α	TCR β	Prediction	Experiment
p15	SLSPRTLNA	9	1–4, 6, 8, 10–12	−2.09	No binding	1.86	No	No
p19	RTLNAWVKV	9	1–6, 8, 10, 12	−0.91	−0.42	−0.66	Yes	Yes
p20	TLNAWVKVI	9	1–4, 6,8, 9, 11, 12	−3.38	−0.5	−0.32	Yes	Yes
p36	EVIPMFSAL	6	1, 2, 4, 7–9	−3.13	0.5	0.02	No	No
p52	DLNMMLNVV	9	1–4, 6, 8, 9, 11, 12	−3.94	1.58	−0.12	No	No
p66	AMQMLKDTI	6	2, 3, 8, 9, 11, 12	−3.39	0.39	0.79	No	No
p79	AEWDRVHPV	8	1–4, 8, 10–12	−0.52	−0.15	2.23	No	No
p104	DIAGTTSTL	5	1, 2, 9, 11, 12	−0.96	−0.01	−0.7	Yes	No
p111	TLQEQIGWM	8	1, 3, 5, 6–8, 11, 12	0.81	3.69	−0.36	No	No
p117	GWMTGNPPV	4	2, 5, 7, 10	−2.89	−0.84	−0.22	Yes	No
p119	MTGNPPVPV	9	1–3, 5, 6, 8, 10–12	−3.13	0.32	0.18	No	No
p129	DIYRRWIIL	4	1, 7, 9, 12	0.39	−1.48	−1.73	No	No
p131	YRRWIILGL	4	2, 5, 10, 12	−3.25	1.09	−0.64	No	No
p134	WIILGLNKI	6	1–3, 8, 9, 12	−1.82	−0.56	−1.16	Yes	No
p135	IILGLNKIV	8	1–3, 5, 8, 9, 11, 12	0.75	0.4	No binding	No	No
p138	GLNKIVRMY	4	1, 2, 9, 11	−2.39	No binding	−0.49	No	No
p141	KIVRMYSPV	8	1–3, 5, 6, 8, 9, 12	−0.91	−0.43	−0.64	Yes	No
p144	RMYSPVSIL	11	1–6, 8–12	−2.34	0.42	2.6	No	No
p165	YVDRFFKCL	7	1–5, 8, 9	−3.21	No binding	−2.32	No	No
p184	NWMTETLLV	4	1, 7, 9, 11	−1.39	0.03	−0.73	No	No
p204	KALGTGATL	7	1–3, 8, 9, 11, 12	−0.84	1.83	−0.74	No	No
p210	ATLEEMMTA	6	1–3, 6, 8, 10	−1.93	−0.46	0.35	No	No
p211	TLEEMMTAC	8	1–3, 6–9, 11	−3.65	1.68	1.24	No	No
p214	EMMTACQGV	8	1–3, 6–9, 11	−1.94	−0.95	−0.06	Yes	Yes

1: ANNPRED; 2: COMPRED; 3: BIMAS; 4: CTLPRED; 5: MHCPRED; 6: NETMHC; 7: PREDEP; 8: PROPRED-I; 9: RANKPED; 10: SMM; 11: SVMHC; 12: SYFPEITHI.