Table 4.

HLA-A2/A0201-restricted T-cell epitopes of SARS-CoV N protein identified by at least 4 epitope prediction methods and molecular docking technique.

Peptide	Sequence	No. of methods	Methods^a	Peptide binding free energy score			Epitope
				MHC	TCR α	TCR β	Prediction	Experiment
n45	GLPNNTASW	5	2, 7, 9, 11, 12	0.01	−0.01	1.89	No	Unknown
n49	NTASWFTAL	4	1, 2, 6, 9	−3.7	1.71	−0.35	No	Unknown
n139	ALNTPKDHI	6	1–3, 9, 11, 12	−2.97	−0.57	−0.28	Yes	Unknown
n159	VLQLPQGTT	4	3, 6, 11, 12	−1.69	−0.12	3.43	No	Unknown
n160	LQLPQGTTL	5	3, 2, 6, 8, 12	−0.59	−0.01	0.5	No	Unknown
n217	ETALALLLL	5	2, 7–9, 12	−3.55	0.13	−0.09	No	Unknown
n219	ALALLLLDR	6	1, 2, 5, 7, 11, 12	−3.07	−0.7	1.76	No	Unknown
n220	LALLLLDRL	7	1–3, 5, 8, 9, 12	−2.4	−0.43	−0.79	Yes	Unknown
n223	LLLDRLNQL	11	1–7, 9–12	−3.57	0.35	−0.34	No	Unknown
n227	RLNQLESKV	9	1–5, 9–12	−3.74	No binding	−0.14	No	Unknown
n263	RTATKQYNV	8	1–4, 6, 10–12	−1.19	−0.35	0.11	No	Unknown
n304	QIAQFAPSA	5	1, 3, 5, 10, 12	−2.74	−0.21	No binding	No	Unknown
n313	SAFFGMSRI	4	1–3, 9	−1.07	−0.58	−0.27	Yes	Unknown
n317	GMSRIGMEV	7	1–3, 5, 6, 10–12	−3.01	0.11	1.44	No	Unknown
n332	LTYHGAIKL	8	1–3, 6, 8–10, 12	−2.53	0.21	−2.18	No	Unknown
n339	KLDDKDPQF	4	5, 7, 9, 11	−2.74	−0.28	−0.3	Yes	Unknown
n352	ILLNKHIDA	6	1, 3, 7, 10–12	−2.6	1.11	−0.09	No	Unknown
n388	KKQPTVTLL	4	1, 2, 6, 9	0.09	No binding	0.26	No	Unknown
n400	DMDDFSRQL	8	1–3, 5, 7, 9, 11,12	−2.34	−0.1	−0.43	Yes	Unknown
n407	QLQNSMSGA	4	1, 3, 10, 11	−1.97	−0.09	1.4	No	Unknown

1: ANNPRED; 2: COMPRED; 3: BIMAS; 4: CTLPRED; 5: MHCPRED; 6: NETMHC; 7: PREDEP; 8: PROPRED-I; 9: RANKPED; 10: SMM; 11: SVMHC; 12: SYFPEITHI.