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. 2015 May 30;69:81–85. doi: 10.1016/j.jcv.2015.05.022

Table 2.

MERS-CoV specific sensitivity results of the first MERS-CoV EQA panel 2014.

Quantitative RT-PCR
Semi-quantitative RT-PCR
Qualitative RT-PCR
Sample no. Origin Dilution Correct results total Correct results Median viral loadb (range) Correct results Median Ct-value (range) Correct results
#13 MERS-CoV 10−2 99.5% 100.0% 7.59 (5.86–9.70) 99.1% 22.2 (16.0–31.2) 100.0%
#9 MERS-CoV 10−3 99.5% 100.0% 6.79 (3.99–8.70) 100.0% 25.1 (15.0–36.5) 97.8%
#2 MERS-CoV 10−4 98.9% 100.0% 5.88 (3.78–7.26) 99.1% 29.0 (22.0–37.7) 97.8%
#5 MERS-CoV 10−4 98.4% 100.0% 5.78 (3.63–7.08) 99.1% 28.9 (23.0–39.7) 95.6%
#6 MERS-CoV 10−5 96.3% 100.0% 4.84 (2.91–7.70) 96.5% 31.7 (18.0–39.5) 93.3%
#10 MERS-CoV 10−6 84.6% 46.2% 3.83 (1.97–5.46) 87.7% 35.5 (28.6–43.6) 73.3%
#8a MERS-CoV 10−7 56.5% 43.3% 2.98 (0.56–6.82) 54.4% 37.7 (32.0–41.5) 51.2%
a

Sample #8 is omitted for further analysis, since the viral load reached the Poisson distribution-level.

b

Viral load in log copies/ml.