Table 2.
MERS-CoV specific sensitivity results of the first MERS-CoV EQA panel 2014.
| Quantitative RT-PCR |
Semi-quantitative RT-PCR |
Qualitative RT-PCR | ||||||
|---|---|---|---|---|---|---|---|---|
| Sample no. | Origin | Dilution | Correct results total | Correct results | Median viral loadb (range) | Correct results | Median Ct-value (range) | Correct results |
| #13 | MERS-CoV | 10−2 | 99.5% | 100.0% | 7.59 (5.86–9.70) | 99.1% | 22.2 (16.0–31.2) | 100.0% |
| #9 | MERS-CoV | 10−3 | 99.5% | 100.0% | 6.79 (3.99–8.70) | 100.0% | 25.1 (15.0–36.5) | 97.8% |
| #2 | MERS-CoV | 10−4 | 98.9% | 100.0% | 5.88 (3.78–7.26) | 99.1% | 29.0 (22.0–37.7) | 97.8% |
| #5 | MERS-CoV | 10−4 | 98.4% | 100.0% | 5.78 (3.63–7.08) | 99.1% | 28.9 (23.0–39.7) | 95.6% |
| #6 | MERS-CoV | 10−5 | 96.3% | 100.0% | 4.84 (2.91–7.70) | 96.5% | 31.7 (18.0–39.5) | 93.3% |
| #10 | MERS-CoV | 10−6 | 84.6% | 46.2% | 3.83 (1.97–5.46) | 87.7% | 35.5 (28.6–43.6) | 73.3% |
| #8a | MERS-CoV | 10−7 | 56.5% | 43.3% | 2.98 (0.56–6.82) | 54.4% | 37.7 (32.0–41.5) | 51.2% |
a
Sample #8 is omitted for further analysis, since the viral load reached the Poisson distribution-level.
b
Viral load in log copies/ml.