Table 3.

Significance levels of compared semi-quantitative RT-PCR data (Ct-values^a) of most used PCR targets.

Target 1 compared to	Target 2	T-test (pb)	F-test (p-valueb)
upE	ORF1a	0.11	0.59
upE	ORF1b	0.00*	0.75
upE	N2	0.30	0.69
upE	N3	0.08	0.95
ORF1a	ORF1b	0.00*	0.48
ORF1a	N2	0.85	0.69
ORF1a	N3	0.62	0.74
ORF1b	N2	0.00*	0.83
ORF1b	N3	0.00*	0.79
N2	N3	0.56	0.95

^*Significant.

^aMedian Ct values (range) upE 28.7 (16.7–41.7); ORF1a 27.65 (17.3–42.9); ORF1b 32.45 (17.3–43.6); N2 28.35 (15.0–37.6); N3 27.3 (16.0–39.0).

^bSignificance levels were calculated without the data of sample 8, since MERS-CoV was not equally distributed.