Table 2.
Sequence of primers and exo-probes for H7N9 RT-RPA assays.
| Name | Sequence 5′–3′ | Amplicon size (nt) |
|---|---|---|
| H7-RPA-FP | CACAGCAAATACAGGGAAGAGGCAATGCAAAATAG | 121 |
| H7-RPA-RP | GAAGTATGAAACATGATGCCCCGAAGCT | |
| H7-RPA-P | CAAAGTATCACATCTT (BHQ1-dT)(THF)(FAM-dT)AGCCGCTGCTTAGTTTGACTGGGTCAATCTG-PH | |
| N9-RPA-FP | CAGAGGGAAACACTCAAACGGAACAATACAC | 129 |
| N9-RPA-RP | CTAGTACTTGACCACCCAATGCATTCCACCCTGC | |
| N9-RPA-P | CTGCTGTTGTACAC(BHQ1-dT)(THF)(FAM-dT)GGGCGGTGATGATAGTGGCCAGCTTATCAG-PH | |
H7-RPA-FP and H7-RPA-RP are forward and reverse RPA primers for the H7 RT-RPA assay; N9-RPA-FP and N9-RPA-RP are forward and reverse RPA primers for the N9 RT-RPA assay; H7-RPA-P and N9-RPA-P are exo-probes; BHQ1-dT, thymidine nucleotide carrying Black Hole Quencher-1; THF, tetrahydrofuran spacer; FAM-dT, thymidine nucleotide-carrying fluorophore; nt, nucleotides.