Table 3.
Results from conventional, TAC and verification testing for samples with discordant results.
| No. of samples (a) | routine testing result | TAC result | verification result |
|---|---|---|---|
| 2 | negative | Adenovirus | Adenovirus |
| 11 | negative | CMV | CMV |
| 1 | negative | CMV | negative |
| 7 | negative | Coronavirus (b) | Coronavirus (b) |
| 3 | negative | hMPV | hMPV |
| 15 | negative | Influenza A | Influenza A |
| 1 | HSV1 | negative (c) | HSV1 |
| 3 | negative | Influenza A (d) | negative |
| 1 | Influenza A (e) | negative | Influenza A (e) |
| 1 | Influenza A (f) | negative | negative |
| 4 | negative | Influenza B | Influenza B |
| 1 | negative | Influenza B (g) | negative |
| 9 | negative | Rhinovirus | Rhinovirus |
| 1 | negative | Rhinovirus (h) | negative |
| 4 | negative | RSV | RSV |
(a) n = 64 (58 samples in total, 6 samples with more than one discordant result).
(b) Coronavirus OC43 (n = 2), Coronavirus 229E (n = 4), Coronavirus NL63 (n = 1).
(c) HSV1 targets not included in TAC assay.
(d) Two samples with only 1/6 and one sample with 3/6 targets for influenza A weakly positive (Ct-value > 30).
(e) Only viral culture positive, verification PCR very weakly positive (Ct-value > 36).
(f) False positive DFA.
(g) Only 1/2 targets weakly positive (Ct-value > 30).
(h) 2/2 Targets for rhinovirus weakly positive (Ct-value > 30).