Fig. 1.

Schematic of the PBMC sample collection and processing. Six aliquots of blood (three for CPT processing and three for LP tube processing) were drawn from three donors. A CPT and LP tube from each donor was processed in parallel by three unique operators. Performance of PBMC isolation methods were measured by their cell yields, viabilities, and recovery both fresh and after cryopreservation and thawing. PBMCs were also stained with surface and intracellular antibodies to assess abundance of cell subsets and functional T cell response following stimulation