Fig. 3.

Similar distribution of immune cell subsets in PBMCs isolated with CPT or LP Tubes. a Gates used to define ten major indicated cell populations in flow cytometry data from one representative PBMC sample (donor 3). b-k Frequencies of cell populations (% of parent gate) in PBMCs isolated by CPT or LP methods determined by flow cytometry for b Lymphocytes, c B cells, d T cells, e CD4+ memory T cells, f CD4+ naïve T cells, g CD4- Naïve T cells, h CD4- memory T cells, i NKT cells, j NK cells, and k monocytes. Note: Lymphocytes used as parent gate for B cells statistics. Horizontal lines indicate mean +/− SEM. Donors 1,2, 3 are depicted by red, black, and blue dots respectively. Three dots are shown per donor which represent single samples processed by three different operators