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. 2020 Mar 30;11:22. doi: 10.1186/s13229-020-00328-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Altered aRG and IP population size in the Mir146^−/− brains. a Sagittal sections from Mir146a^+/+and Mir146a^−/− brains at E14.5 labeled with PAX6, TBR2, and merge. b Boxplot showing the number of PAX6⁺ cells (±S.D.) counted using 3 images from comparable plane per mouse. c Boxplot showing the quantification of TBR2⁺ cells (±S.D.) from the same images as b. d Boxplot showing the ratio of TBR2⁺/PAX6⁺ cells. e Phospho-histone 3 (PH3) stains dividing cells in the neocortex at E14.5 (white arrows). f Graph showing the average number of PH3⁺ cells (±S.D.) in the SVZ. *P < 0.05 by Student’s unpaired 2-tailed t test. g BrdU staining in the neocortex at E14.5 after 2h pulse chase; images were divided into five equal bins for counting. h Percentage of BrdU+ cells (±S.D.) per bin (normalized against the total number of labeled cells). NS not significant; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 by Mann-Whitney U test