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. 2020 Mar 30;18:51. doi: 10.1186/s12964-020-00557-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Wnt5a up-regulates IL-10 expression by activating CaKMII-ERK1/2-STAT3 pathway. a Western blot analysis of p-ERK1/2, p-STAT3 and p-NF-κB p65 expression in M0 macrophages with or without Wnt5a. b Immunofluorescence staining for ERK1/2 and STAT3 in M0 macrophages with or without Wnt5a. Bar = 25 μm. c Western blot analysis of IL-10, p-ERK1/2 and p-STAT3 expression in Wnt5a-treated M0 macrophages with or without U0126 or S3I-201. d Flow cytometry analysis of Wnt5a-treated M0 macrophages with or without U0126 or S3I-201. Error bars, SEM. e ELISA analysis of IL-10 secretion level in Wnt5a-treated M0 macrophages with or without U0126 or S3I-201. Error bars, SEM. f Western blot analysis of Wnt5a-treated M0 macrophages with or without CK59 or U0126 or S3I-201. g ELISA analysis of IL-10 secretion level in Wnt5a-treated M0 macrophages with or without CK59. Error bars, SEM. h Flow cytometry analysis of Wnt5a-treated M0 macrophages with or without CK59. Error bars, SEM. All experiments were performed independently at least three times. Statistical analysis was conducted using one-way ANOVA. ***P < 0.001