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. 2020 Mar 30;18:51. doi: 10.1186/s12964-020-00557-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Wnt5a indirectly promotes tumor proliferation, migration and invasion through M2 macrophages. a Flow chart of CRC cells co-cultured with M0 macrophages treated with Wnt5a or Wnt5a + IL-10 antibody or Wnt5a + IgG. b, c Representative photographs and quantifications of clone formation assay in HCT116 and DLD-1 cells co-cultured with conditioned M0 macrophages. Bar = 0.5 cm. d, e Transwell migration assay of HCT116 and DLD-1 cells co-cultured with conditioned M0 macrophages. Bar = 200 μm. f, g Transwell invasion assay of HCT116 and DLD-1 cells co-cultured with conditioned M0 macrophages. Bar = 200 μm. All experiments were performed in triplicate. Statistical analysis was conducted using one-way ANOVA. Error bars, SEM. ns, not significant. ***P < 0.001