Fig. 1.

Effects of high-dose ibrutinib on the release of proinflammatory cytokines and anti-Scl-70. B cells were treated with ibrutinib (10 μM) and stimulated with CpG (1 μM); DMSO was used as control. a Supernatants of B cell cultures were analyzed for cytokine levels in a multiplex assay after 24 h of culture (n = 10). b Supernatants of B cell cultures were analyzed for anti-Scl-70 levels via ELISA after 72 h of culture (n = 5). Bars represent the mean. Error bars indicate SEM. *p < 0.05, **p < 0.01, ***p < 0.001