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. 2020 Mar 30;21:22. doi: 10.1186/s12860-020-00267-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — a. Quantification of fibrillar collagen deposited in MEF Hsp47−/− and Hsp47 +/+cells (control) at 24 h after H₄₇ treatment (0.5 μM) using Sirius Red assay. b. Quantification of deposited COL I, III, IV, V and XII from immuno staining assays in MEF Hsp47−/− cultures. Error bars representing standard deviation from n-3 experiments in a and c, The plots in both a and c assays were normalized by MEF Hsp47−/− cells untreated condition taken as 1. Statistical significance in a and c was analyzed by Tukey test. Significance was calculated by comparing non treated against Hsp47 treated cells (mean ± SD, ANOVA, *** p < 0.001). c. Western blot of COL I, III, IV, V and XII in deposited collagen from MEF Hsp47 +/+, Hsp47 −/− and Hsp47−/− cultures 24 h after treatment with 0.5 μM H₄₇. Equal amount of cells were analyzed. Black bands indicate signal from collagen subtypes specific antibody. The wiskers plot indicates relative gel bands intensity of different collagen types in MEF Hsp47 +/+, Hsp47 −/− and Hsp47−/− H₄₇ treated conditions. Error bar indicates standard deviation from n-3 experiments. Statistical significance was analyzed by Tukey test. Significance was calculated by comparing non treated Hsp47 −/− against Hsp47 treated cells (mean ± SD, ANOVA, *** p < 0.001)